Legal Question in Criminal Law in India

28.04.2012

Dear Sir / Madam,

ABO blood type of semen could be determined by identifying a protein with molecular mass of 84 kDa using an electro-phoretic method (7). Although this procedure enables a seminal BGS with a molecular mass of 84 kDa to be identified visually, intricate procedures involving immunoblotting with anti-A, B and H mAbs followed by immunoprecipitation are required, making it unsuitable for routine use in the forensic laboratory. The sandwich ELISA for detecting the ABO blood type of semen is simple and much easier to perform than the electro-phoretic procedure.

p 84 is present not only in semen samples from secretors (Se), but also in those from non-secretor (se). However, BGS from p 84 cannot be detected in semen from the latter, which suggests strongly that seminal p 84 from non-secretors does not bear ABH sugar epitopes. Therefore, the content of p 84 in semen should be examined by an indirect ELISA using the antip84 mAb before ABO blood typing of semen by the sandwich

ELISA.

This sandwich ELISA is used widely in many forensic laboratories for determining the ABO

blood type of semen which is contaminated with vaginal secretions, saliva or blood occurs frequently in cases of sexual assault. Therefore, anti-p 84 mAb's are supplied to researchers who wish to use it to study the molecular properties of

p 84 by analyzing its DNA sequence.

As illustrated above, even if the ABO grouping of the semen sample is found inconclusive, the same sample can be used for its DNA profiling.

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Regards,

Check with doctor/forensic expert.